Screening and identification of genes affecting grain quality and spikelet fertility during high-temperature treatment in grain filling stage of rice

Abstract Background Recent temperature increases due to rapid climate change have negatively affected rice yield and grain quality. Particularly, high temperatures during right after the flowering stage reduce spikelet fertility, while interfering with sugar energy transport, cause severe damage quality by forming chalkiness grains. The effect of high-temperature on fertility filling was evaluated using a double haploid line derived from another culture F 1 crossing Cheongcheong Nagdong cultivars. Quantitative trait locus (QTL) mapping identifies candidate genes significantly associated at temperatures. Results Our analysis screened OsSFq3 that contributes high-temperature. fine-mapped in region RM15749-RM15689 chromosome 3, wherein four related synthesis decomposition amylose, starch component, were predicted. Four major genes, including , 10 different involved amylose amylopectin, which are constituents, together relative expression levels analyzed. highly expressed initial treatment. It exhibited homology FLOURY ENDOSPERM 6 Gramineae plants is therefore expected function similarly. Conclusion QTL, identified herein could be effectively used breeding varieties improve quality, tolerating temperatures, cope changes. Furthermore, linked markers can aid marker-assisted selection high-quality -yield tolerant